IL-5 anticorps (Biotin)

N° du produit ABIN116077

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-5 dans WB et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour IL-5 anticorps (Biotin) (ABIN116077)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-5 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IL-5 anticorps (Biotin)

Specificité: This antibody recognizes Human IL-5.

Purification: Affinity Chromatography.

Immunogène: Highly pure (>98%) E.coli derived recombinant hIL-5 (human IL-5).

Information d'application

Indications d'application: ELISA: To detect hIL-5 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-5 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur IL-5

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: Interleukin-5 / IL5

Sujet: Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF

ID gène: 3567

NCBI Accession: NP_000870

UniProt: P05113

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour